Composite
Part:BBa_K3158005:Design
Designed by: Shiyuan Li Group: iGEM19_Xiamen_City (2019-10-15)
J23100-RBS-bpul-ter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 184
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 551
Illegal NgoMIV site found at 1019
Illegal AgeI site found at 307
Illegal AgeI site found at 1184 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence should express bpul protein.
Source
Laccase from Bacillus pumilus