Composite

Part:BBa_K3158005:Design

Designed by: Shiyuan Li   Group: iGEM19_Xiamen_City   (2019-10-15)


J23100-RBS-bpul-ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 184
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 551
    Illegal NgoMIV site found at 1019
    Illegal AgeI site found at 307
    Illegal AgeI site found at 1184
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence should express bpul protein.


Source

Laccase from Bacillus pumilus

References